Covalent Modification of Protein Kinase C Isozymes by the Inactivating Peptide Substrate Analog N-Biotinyl-Arg-Arg-Arg-Cys-Leu-Arg-Arg-Leu

Preferential ADP-ribosylation of arginine-3 in synthetic heptapeptide Leu-Arg-Arg-Ala-Ser-Leu-Gly.

Hen liver nuclear ADP-ribosyltransferase modified the synthetic heptapeptide Kemptide (Leu-Arg-Arg-Ala-Ser-Leu-Gly) at arginine-2 and/or arginine-3. Trypsin treatment of ADP-ribosyl-Kemptide revealed that the ADP-ribosylation of arginine-3 was constantly more abundant than that of arginine-2. ADP-ribosylation of Kemptide suppressed the subsequent phosphorylation by cyclic AMP-dependent protein ...

Renal autoimmune epitope of group A streptococci specified by M protein tetrapeptide Ile-Arg-Leu-Arg.

The renal glomerular cross-reactivity of the amino-terminal region of type 1 streptococcal M protein was investigated. Antisera raised in rabbits against a synthetic peptide representing residues 1-26 and a peptide from which residues 20-22 had been omitted during synthesis were capable not only of opsonizing type 1 streptococci but also of reacting in immunofluorescence tests with human renal ...

Phosphorylation of acyl and dansyl derivatives of the peptide Leu-Arg-Arg-Ala-Ser-Leu-Gly by the cAMP-dependent protein kinase.

A series of acyl derivatives and a dansyl derivative of the synthetic peptide Leu-Arg-Arg-Ala-Ser-Leu-Gly have been tested as substrates for the catalytic subunit of the CAMP-dependent protein kinase (ED 2.7.1.37; ATP: phosphotransferase). The purpose of this study was to test whether substitution of the NH2-terminal leucine with acyl substituents of varying chain length and hydrophobicity woul...

Technetium-99m- Arg-Arg-Leu(g2), a modified peptide probe targeted to neovascularization in molecular tumor imaging.

PURPOSE The (131)I-tRRL small peptide probe has been identified in our previous study as a robust tumor molecular radiopharmaceutical that specifically binds to tumor-derived endothelial cells. In this study we developed a smaller structure cyclic tRRL (g2) radiolabeled with (99m)Tc as a novel and optimized peptide probe on tumor angiogenesis molecular imaging. METHODS Both tRRL (g2) and cont...

RF 3 Leu Leu Leu Arg - X - - X - Leu Hydrophobic Termination